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By Kurt E. Johnson

ISBN-10: 0683062107

ISBN-13: 9780683062106

The part on phone biology has been significantly improved and now comprises info on either the structural and useful points of the subject.

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It should also be noted if the biopsy specimen is too small, or is composed largely of cortical bone 39 and subcortical marrow, or shows crush artefact or other artefactual distortion of the architecture. Focal lesions, such as granulomas or infiltrates of metastatic tumour or lymphoma, are often better appreciated on low power examination. Following this, the bone, haemopoietic elements and marrow stromal elements should be studied using medium power (×10 or ×20 objective) and a high power dry objective (×40 or ×60); examination under oil immersion (×100 objective) is not necessary as a routine but is often useful to study fine cytological detail.

The latter are small, proliferative cells with diploid nuclei, not generally recognizable in normal bone marrow. In normal marrow, the earliest morphologically recognizable cell in the megakaryocyte lineage is the megakaryocyte itself although, when haemopoiesis is abnormal, mega- TH E N OR M A L B ON E M A R R OW 21 Fig. 28 Section of normal BM: a macrophage containing cellular debris. Resin-embedded, H&E ×100. Fig. 29 Section of trephine biopsy specimen showing a network of dendritic macrophages.

61 [54]. Percentage of intertrabecular space occupied by different cell types TH E N OR M A L B ON E M A R R OW 100 35 Other 90 80 Lymphocytes 70 60 50 40 30 Myeloid (granulocytic) 20 Glaser et al. (1950) 10 Gairdner et al. (1952) 1 2 3 4 Erythroid 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Age (months) Fig. 52 The percentage of the intertrabecular space occupied by cells of different lineages during the first 18 months of life, derived from Rosse et al. [38]; the higher initial erythroid percentage and sharp fall in erythroblast number observed by Glaser et al.

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Histology and Cell Biology by Kurt E. Johnson

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